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KMID : 1177720080020020015
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Journal of Alternatives to Animal Experiments
2008 Volume.2 No. 2 p.15 ~ p.19
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Vancomycin-resistant enterococci from a zebrafish gut
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Seok Seung-Hyeok
Baek Min-Won
Kim Dong-Jae
Na Yi-Rang
Han Ju-Hee
Koh Ae-Sun
Jung Woo-Kyung
Park Jae-Hak
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Abstract
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The use of zebrafish embryos is not regulated by current Animal Protection law (full amendment, January 26, 2007) in Korea and therefore considered as a refinement, if not replacement of animal experiments because the complicated physiological environment is absent in vitro system. The skin ulcers and abdominal swelling were observed in laboratory maintained zebrafish. Contents of abdominal swelling found at necropsy are peritoneal exudates. Vancomycin resistant enterococci (VRE) were detected for VanC-1 (Enterococcus gallinarum) by PCR on cultured colonies from the affected fish gut, exudates. In addition, unhatched eggs were observed in the abdominal exudates, based upon the H&E staining. This is the first documentation of the precise identification of VRE with VanC-1 gene in laboratory-maintained zebrafish. The pathogenesis to VRE infection in zebrafish was required in the following study.
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KEYWORD
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Zebrafish, vancomycin resistant enterococci, VRE, animal welfare
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